The aim of the present paper is to establish the detailed numerical results for bioconvection boundary layer flow of a two-phase dusty nanofluid. The dusty fluid contains gyrotactic microorganisms along an isothermally heated vertical wall. The physical mechanisms responsible for the slip velocity between the dusty fluid and nanoparticles, such as thermophoresis and Brownian motion, are included in this study. The influence of the dusty nanofluid on heat transfer and flow characteristics are investigated in this paper. The governing equations for two-phase model are non-dimensionalized and then solved numerically via two-point finite difference method together with the tri-diagonal solver. Results are presented graphically for wall skin friction coefficient, rate of heat transfer, velocity and temperature profiles and streamlines and isotherms. To ensure the accuracy, the computational results are compared with available data and are found in good agreement. The key observation from the present analysis is that the mass concentration parameter, D-rho, extensively promotes the rate of heat transfer, Q(w), whereas, the wall skin friction coefficient, tau(w), is reduced by loading the dust parameters in water based dusty nanofluid. (C) 2017 Elsevier Ltd. All rights reserved.