The gamma-cyclodextrin-graphite oxide-carbon nanotube (gamma CD-GO-CNT) composite has shown the best capability and sensitivity of supramolecular recognition compared to the other two CD-GO-CNT composites (aCD-GO-CNT and beta CD-GO-CNT) in our previous study. The biggest CD (gamma CD) was very important in the formation of the supramolecular complex and made the detection of the biomolecules easier. In this report, significantly improved supramolecular recognition capability of gamma CD-GO-CNT is introduced by using thermally reduced GO (TRGO), instead of typical GO. New synthesized gamma CD-TRGO-CNT composite film shows lower electrochemical impedance, higher carbon to oxygen atomic ratio, and larger surface area compared to the gamma CD-GO-CNT composite film, resulting in higher sensitivity and capability in the supramolecular recognition. The sensitivity, defined as the slope of the maximum response current versus guest molecule concentration graph, increases significantly from 4.42 to 9.44 for dopamine and from 2.21 to 4.15 for ascorbic acid. The excellent enhancement of the supramolecular recognition of the gamma CD-TRGO-CNT composite is attributed to TRGO. It is, therefore, concluded that it is very important to load gamma CD in the composite as well as to decrease the electrochemical impedance for effective electrochemical biosensors. (C) 2017 Elsevier Ltd. All rights reserved.