The rationally designed sensor architecture is very important to improve the sensitivity and selectivity for H2O2 enzyme-free electrochemical sensor. In this work, a sensitive H2O2 biosensor was fabricated by electrochemical deposition of Au-Pt bimetallic nanoparticles (NPs) on molybdenum disulfide nanoflowers (MoS2 NFs). Au-Pt NPs was dispersed or stabilized by the effective support matrix of MoS2 nanosheets, which was effectively enhance the conductivity, catalytic performance and long-term stability. The experimental results show that MoS2-Au/Pt nanocomposites exhibit excellent catalytic activity for specific detection of H2O2, and electrochemical measurement results show that the enzyme-free electrochemical sensor has large linear range of 10 mu M to 19.07 mM with high sensitivity of 142.68 mu A mM(-1) cm(-2.) This novel sensor produced satisfactory reproducibility and stability, and exhibited superior potential for the practical quantitative analysis of H2O2 in serum samples. (C) 2017 Published by Elsevier Ltd.