A superhydrophobic and self-cleaning photoluminescent film is developed by creating hierarchical micro-/nano-structures on soy protein film surface with hydrophobic nanoparticles. The functional protein film can resist strong acid/alkaline, organic solvents and slight mechanical abrasion without losing light-emitting and self-cleaning properties. Such film can be applied in some extreme environments for long-term outdoor service. (C) 2017 Elsevier B.V. All rights reserved.