Both alpha-ketoglutarate (alpha-KG) and pyruvate (PYR) are important organic acids with promising applications in the food, pharmaceutical and chemical industries. During the production of alpha-KG by different microorganisms, PYR is always present as a by-product. Strategies have been applied to eliminate PYR accumulation since it can bring difficulties to the downstream separation process. However, modern separation technologies have already conquered this problem. Therefore, this study was aimed at simultaneously enhancing alpha-KG and PYR production by Yarrowia lipolytica WSH-Z06. Using a fed-batch strategy, in which the initial glycerol concentration was 50 g.L-1, the residual glycerol concentration was maintained 2030 g.L-1 by constant feeding at a rate of 1.25 g.L-1.h(1). The titers of alpha-KG and PYR were increased by 9.6% and 176.8%, and reached 67.4 g.L-1 and 39.1 g.L-1, respectively. The final yield of keto acids was 0.71 g.g(1) glycerol, which is 42.0% higher than that of the optimal batch fermentation. (C) 2017 Elsevier Ltd. All rights reserved.