We report the association of Cristulariella moricola (Hino) Redhead with a zonate leaf spot on Eucalyptus benthamii (Myrtaceae). We observed the disease outbreak across a series of experimental E. benthamii plantings in Barnwell County, South Carolina, USA in October 2015. The disease caused nearly complete foliar necrosis of most trees in one experimental planting, but disease incidence remained at low levels (<10% of foliage affected in most trees) in other plantings. Disease development coincided with periods of extended rainfall, which has also been observed in outbreaks of zonate leaf spot caused by C. moricola in other tree species. (C) 2017 Elsevier Ltd. All rights reserved.