tRNA maturation involves several steps, including processing, splicing, CCA addition, and post transcriptional modifications. tRNA(His) guanylyltransferase (Thgl) is the only enzyme known to catalyze templated nucleotide addition in the 3'-5' direction, unlike other DNA and RNA polymerases. For a better understanding of its unique catalytic mechanism at the molecular level, we determined the crystal structure of GTP-bound Thgl from Saccharomyces cerevisiae at the maximum resolution of 3.0 angstrom. The structure revealed the enzyme to have a tetrameric conformation that is well conserved among different species, and the GTP molecule was clearly bound at the active site, coordinating with two magnesium ions. In addition, two flexible protomers at the potential binding site (PBS) for tRNA(His) were observed. We suggest that the PBS of the tetramer could also be one of the sites for interaction with partner proteins. (C) 2017 Elsevier Inc. All rights reserved.