Background: The aim of this study was to analyze the effect of micro strain stress on the proliferation of endothelial progenitor cells in vitro by the MAPK-ERK1/2 signaling pathway. Material and methods: In this study, we report the effect of micro strain stress on proliferation of endothelial progenitor cells (EPCs) in vitro by loading different types of micro strain stress on EPCs. We also explore the possible mechanisms of this phenomenon. Results: The results of flow cytometry show that appropriate micro strain stress can promote EPC proliferation. This phenomenon coincides with the upregulation of multiple genes or proteins affecting cell proliferation, including Cyclin D1, Proliferating Cell Nuclear Antigen (PCNA) and phospho-ERK1/2. Conclusion: The results collectively suggest that proper mechanical stimulation can promote the proliferation of EPCs and promote angiogenesis. (C) 2017 Published by Elsevier Inc.