In this work, a variety of hyperbranched polymers (HBPs), such as hyperbranched polycarbonates, polyesters, polyurethanes and polyacetals, was successfully synthesized from castor oil and soybean oil based monomers via a A(2)+B-3 polycondensation. First, B-3 monomer triols (TriOL), trialdehydes (TriAD), and tricarboxylic acids (TriAC) were obtained by ozonolysis of castor oil and soybean oil with following reductive or oxidative treatment. Their structures were characterized by H-1 NMR and ATR-FTIR spectroscopy as well as electrospray ionization-Time of Flight-mass spectrometry. These trifunctional B-3 monomers were applied in the preparation of HBPs. The resulting HBPs had number averaged molar mass (M-n) up to 9400 g/mol and weight averaged molar mass (M-w) up to 40,000 g/mol. Through adjusting the initial molar ratio of A(2) to B-3 monomers, hydroxyl terminated (from TriOL monomers) or carboxylic acid (from TriAC monomers) terminated HBPs could be obtained. All the HBPs were characterized by H-1 NMR, size exclusion chromatography, and DSC. These HBPs are potential candidates for the synthesis of cross-linked polymeric materials or in biomedical applications. (c) 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 2104-2114