Hypothesis: The physicochemical properties of solid lipid nanoparticles (SLN) depend on lipid compositions. An addition of secondary solid complex triglycerides (Softisan 378; 5378) into solid wax (cetyl palmitate; CP) is expected to influence the properties of obtained SLN compared to SLN prepared from sole CP. Experiments: Ibuprofen-loaded SLN (IBSLN-TG) composed of different ratios of CP and 5378 were prepared and evaluated in term of size, zeta potential (ZP), entrapment efficiency (E.E.), crystallinity, lipid-drug interaction and in vitro drug release. Findings: After production, all developed IBSLN-TG prepared from different ratios of CP and S378 had the particle size in the nanometer range (180-200 nm) with the ZP values of higher than vertical bar-40 mV vertical bar and possessed approximately 100% E.E. The release of IBSLN-TG demonstrated the biphasic pattern with a fast release followed by sustained release, which was fitted to Higuchi's kinetics. The addition of S378 into CP-matrix led to a slight decrease in particle size and surface charge, and distortion of CP crystallization. The results from H-1-NMR indicated the formation of tiny liquid S378 nanocompartments within CP-matrix. The localization of ibuprofen in the 5378 nanocompartments and the interaction between ibuprofen and 5378 had an impact on the release profiles of IBSLN-TG depending on the ratios of CP and 5378. (C) 2017 Elsevier Inc. All rights reserved.