A simple effective and environmentally friendly pathway to form graphene modified bio-cathode was induced by polarity reversion of graphene modified bioanode, which was made by in situ microbial-induced reduction of graphene oxide (GO). Graphene assembled by microbes showed that D/G intensity increased from 0.84 to 0.91 and C/O atomic ratio added from 2.13 to 4.45, indicating GO was reduced largely. The graphene modified bio-cathode exhibited crumples that grown on the lamellar and glossy surface and was capable of improving catalytic reduction of oxygen. Microbial fuel cell (MFC) fabricated with graphene modified biocathode obtained 1.22 times in maximum power density, 0.21 times in interfacial charge transfer resistance, and recorded an obvious redox peaks at -0.50 V (vs. SCE) than control biocathode MFC. Geobacter, Clostridium, Pseudomonas, Geothrix and Hydrogenophaga belonged to exoelectrogens occupied 17.53% in graphene modified biocathode, 2.07% in control biocathode in genus level. This study provided a new insight into the feasibility to make microbes self-assembled graphene to improve electrochemical performance of biocathode MFC. (C) 2017 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.