Lignocellulosic biomass is considered as a major feedstock to produce value-added renewable chemicals. In this study, a new water-based lubricant was prepared using biomass derived levulinic acid (LA) and polyols such as ethylene glycol and glycerol. The products were separated by rotary film molecular distillation and characterized by '14 NMR and mass spectrometry. Lubricant properties such as kinematic viscosity, pour, cloud, and flash points, copper strip corrosion, and volatility at 120 degrees C were evaluated according to standard ASTM methods. Furthermore, the hydrolytic stability and tribological properties of the products were tested for water-based lubricants. The results indicated that glycerol ester of levulinic acid (LAGLE) exhibited superior lubricant properties, strong resistance to hydrolytic degradation, and excellent antiwear performance, implying that the biomass-derived LAGLF, was a potential water-based lubricant.