This work concerns the investigation of the sequential production of lactic acid (LA) and biogas from food waste (FW). LA was produced from FW using a Streptococcus sp. strain via simultaneous saccharification and fermentation (SSF) and separate enzymatic hydrolysis and fermentation (SHF). Via SHF a yield of 0.33 g(LA)/gFW (productivity 3.38 g(LA)/L.h) and via SSF 0.29 g(LA)/g(FW) (productivity 2.08 g(LA)/L.h) was obtained. Fermentation residues and FW underwent anaerobic digestion (3 wt% TS). Biogas yields were 0.71, 0.74 and 0.90 Nm(3)/kg(VS) for FW and residues from SSF and SHF respectively. The innovation of the approach is considering the conversion of FW into two different products through a biorefinery concept, therefore making economically feasible LA production and valorising its fermentative residues. Finally, a mass balance of three different outlines with the aim to assess the amount of LA and biogas that may be generated within different scenarios is presented. (C) 2017 Elsevier Ltd. All rights reserved.