Synthesis of Conjugated Polymer Nanoparticles with Core-Shell Structure for Cell Imaging and Photodynamic Cancer Therapy

Choongho Kim; Sun-Young Kim; Yong Taik Lim; Taek Seung Lee

Macromolecular Research, Vol.25, No.6, 572-577, June, 2017

DOI10.1007/s13233-017-5104-y Export Citation

Synthesis of Conjugated Polymer Nanoparticles with Core-Shell Structure for Cell Imaging and Photodynamic Cancer Therapy

Choongho Kim, Sun-Young Kim¹, Yong Taik Lim¹, and Taek Seung Lee^†

Organic and Optoelectronic Materials Laboratory, Department of Organic Materials Engineering, Chungnam National University, Daejeon 34134, Korea
¹SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi 16419, Korea

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Conjugated polymer nanoparticles were successfully capped with a commercially available triblock copolymer Pluronic F127® to form stable nanoparticles with the potential for photodynamic therapy (PDT) of cancer cells. PDT is known as an effective, simple treatment method without the need for surgery. Investigations on the particle structure revealed that the nanoparticles were fabricated with a core-shell structure of ~192 nm. The PDT effects of the nanoparticles on HeLa cancer cells were investigated via cell imaging and cytotoxicity tests. The nanoparticles were biocompatible and were easily internalized by HeLa cells. Moreover, the nanoparticles efficiently generated reactive oxygen species to kill cancer cells under UV irradiation. Therefore, the nanoparticles have promising applications for cancer cell imaging and therapy.

Keywords:conjugated polymer nanoparticles;cancer cells;imaging;photodynamic therapy;reactive oxygen species

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[Referenced By]

[1] Folkman J, Nat. Med., 1, 27 (1995)

[2] Anand P, Kunnumakara AB, Sundaram C, Harikumar KB, Tharakan ST, Lai OS, Sung B, Aggarwal BB, Pharm. Res., 25, 2097 (2008)

[3] Newell SA, Girgis A, Sanson-Fisher RW, Savolainen NJ, Hons B, Am. J. Prev. Med., 17, 211 (1999)

[4] Lin J, Wang S, Huang P, Wang Z, Chen S, Niu G, Li W, He J, Cui D, Lu G, Chen X, Nie Z, ACS Nano, 7, 5320 (2013)

[5] Paszko E, Ehrhardt C, Senge MO, Kelleher DP, Reynolds JV, Photodiagnosis Photodyn. Ther., 8, 14 (2011)

[6] Dolmans DEJGJ, Fukumura D, Jain RK, Nat. Rev. Cancer, 3, 380 (2003)

[7] Ku EB, Oh KT, Youn YS, Lee ES, Macromol. Res., 24(7), 634 (2016)

[8] Castano AP, Mroz P, Hamblin MR, Nat. Rev. Cancer, 6, 535 (2006)

[9] Peterson CM, Lu JM, Sun Y, Peterson CA, Shiah J, Straight RC, Kopecek J, Cancer. Res., 56, 3980 (1996)

[10] Khdair A, Chen D, Patil Y, Ma L, Dou QP, Shekhar MPV, Panyam J, J. Control. Release, 141, 137 (2010)

[11] Son KJ, Yoon H, Kim J, Jang W, Lee Y, Koh W, Angew. Chem.-Int. Edit., 50, 11968 (2011)

[12] Kang SH, Nafiujjaman M, Nurunnabi M, Li L, Khan HA, Cho KJ, Huh KM, Lee YK, Macromol. Res., 23(5), 474 (2015)

[13] Pecher J, Mecking S, Chem. Rev., 110(10), 6260 (2010)

[14] Zhu CL, Liu LB, Yang Q, Lv FT, Wang S, Chem. Rev., 112(8), 4687 (2012)

[15] Kim D, Lee TS, ACS Appl. Mater. Interfaces, 8, 34770 (2016)

[16] Jin YJ, Oh JH, Kwak G, Polym. Korea, 40(5), 736 (2016)

[17] Feng L, Zhu C, Yuan H, Liu L, Lv F, Wang S, Chem. Soc. Rev., 42, 6620 (2013)

[18] Duncan R, Nat. Rev. Cancer, 6, 688 (2006)

[19] Feng L, Zhu J, Wang Z, ACS Appl. Mater. Interfaces, 8, 19364 (2016)

[20] Chemburu S, Corbitt TS, Ista LK, Ji E, Fulghum J, Lopez GP, Ogawa K, Schanze KS, Whitten DG, Langmuir, 24(19), 11053 (2008)

[21] Xing CF, Liu LB, Tang HW, Feng XL, Yang Q, Wang S, Bazan GC, Adv. Funct. Mater., 21(21), 4058 (2011)

[22] Ji E, Corbitt TS, Parthasarathy A, Schanze KS, Whitten DG, ACS Appl. Mater. Interfaces, 3, 2820 (2011)

[23] Zhu CL, Yang Q, Lv FT, Liu LB, Wang S, Adv. Mater., 25(8), 1203 (2013)

[24] Yuan Y, Liu J, Liu B, Angew. Chem.-Int. Edit., 126, 7291 (2014)

[25] Li S, Chang K, Sun K, Tang Y, Cui N, Wang Y, Qin W, Xu H, Wu C, ACS Appl. Mater. Interfaces, 8, 3624 (2016)

[26] Gao D, Agayan RR, Xu H, Philbert MA, Kopelman R, Nano Lett., 6, 2383 (2006)

[27] Li K, Liu B, J. Mater. Chem., 22, 1257 (2012)

[28] Yu JB, Wu CF, Sahu SP, Fernando LP, Szymanski C, McNeill J, J. Am. Chem. Soc., 131(51), 18410 (2009)

[29] Zhou X, Liang H, Jiang P, Zhang KY, Liu S, Yang T, Zhao Q, Yang L, Lv W, Yu Q, Huang W, Adv. Sci., 3, 150015 (2016)

[30] Shi HF, Ma X, Zhao Q, Liu B, Qu QY, An ZF, Zhao YL, Huang W, Adv. Funct. Mater., 24(30), 4823 (2014)

[31] Pitto-Barry A, Barry NPE, Polym. Chem., 5, 3291 (2014)

[32] Kabanov AV, Batrakova EV, Alakhov VY, J. Control. Release, 82, 189 (2002)

[33] Kabanov AV, Batrakova EV, Miller DW, Adv. Drug Deliv. Rev., 55, 151 (2003)

[34] Kabanov AV, Lemieux P, Vinogradov S, Alakhov V, Adv. Drug Deliv. Rev., 54, 223 (2002)

[35] Oktavia L, Kim SJ, Kim JH, Park H, Lee PCW, Kwak M, Polym. Korea, 40(1), 130 (2016)

[36] Yang RQ, Tian RY, Yan JG, Zhang Y, Yang J, Hou Q, Yang W, Zhang C, Cao Y, Macromolecules, 38(2), 244 (2005)

[37] Hou Q, Xu Y, Yang W, Yuan M, Peng J, Cao Y, J. Mater. Chem., 12, 2887 (2002)

[38] Hu Z, Zhao F, Wang Y, Huang Y, Chen L, Li N, Li J, Lia Z, Yi G, Chem. Commun., 50, 10815 (2014)

[39] Chompoosor A, Saha K, Ghosh PS, Macarthy DJ, Miranda OR, Zhu Z, Arcaro KF, Rotello VM, Small, 6, 2246 (2010)

[40] Jang G, Seo S, Lee TS, Sens. Actuators B-Chem., 221, 1229 (2015)

[41] Klarner G, Lee JI, Davey MH, Miller RD, Adv. Mater., 11(2), 115 (1999)

[42] Hennebicq E, Pourtois G, Scholes GD, Herz LM, Russell DM, Silva C, Setayesh S, Grimsdale AC, Mullen K, Bredas JL, Beljonne D, J. Am. Chem. Soc., 127(13), 4744 (2005)

[43] Ariu M, Sims M, Rahn MD, Hill J, Fox AM, Lidzey DG, Phys. Rev. B, 67, 195333 (2003)