This study investigated the effect of the precipitation time and temperature on the efficiency of fractional precipitation for the purification of (+)-dihydromyricetin, and analyzed the kinetics and thermodynamics of the fractional precipitation process. The time required to obtain a given yield was the shortest at 277 K in a time-temperature-transformation (TIT) diagram. When the Johnson-Mehl-Avrami-Komolgorov (JMAK) equation was applied to experimental data, simultaneous mechanisms of nucleation and growth were determined at precipitation temperatures of 277, 280, and 283 K, while consecutive mechanisms of nucleation and growth were determined at 263 K and 291 K. A thermodynamic analysis showed that the enthalpy change (Delta H degrees) and entropy change (Delta S degrees) were both negative, plus the Gibbs free energy change (Delta G degrees) was negative and decreased when decreasing the temperature (283, 280, and 277 K). Thus, the precipitation was more feasible when using a lower temperature, and the results indicated that the fractional precipitation process for purifying (+)-dihydromyricetin was exothermic, irreversible, and spontaneous. (C) 2016 Elsevier Ltd. All rights reserved.