In the present work, the formation of TiO2 nanostructures by anodization was investigated. The anodic reaction was carried out in phosphate/glycerol electrolyte at high temperatures. By controlling the chemical and electrical parameters of anodization, TiO2 nanostructures with various morphologies can be formed. Additionally, chemical analysis of electrolytes provide the clue to a possible anodization reaction pathway. (C) 2016 The Electrochemical Society. All rights reserved.