Silver nanoparticles (Ag NPs) embedded and stabilized with polyethylene glycol (PEG) were synthesized as colloids by heating and exposure to sunlight (direct and indirect) irradiation as green method. The deposition of Ag NP-PEGs onto Si-wafers was also made using the electrospray ionization deposition technique. The generating of Ag NP-PEGs as colloids was examined by UV-visible spectroscopy (UV-Vis) and transmission electron microscopy (TEM). The chemical composition of the resulted nanocomposites was evaluated by Fourier transform infrared (FTIR) and that of thin-film surfaces by X-ray photoelectron spectroscopy. Structure-property relationships of Ag-PEG nanocomposites prepared by heating were discussed in dependence on the time of heating. The UV-visible results confirmed the successful synthesis of spherical Ag NPs with absorption peaks at a wavelength of lambda = 413 nm for the heating method and at lambda = 418 as well as 449 nm for direct and indirect exposure to the sunlight. AgPEG nanocomposite thin films showed excellent antimicrobial activity. These results revealed that the Ag-PEG nanocomposites thin films can be used as potential materials in biomedical applications.