Antimicrobial three dimensional (3D) woven fabric filters are fabricated by wrapping the weft yarn with electrospun nanofibers containing 2 wt% silver nanoparticles (AgNPs). For comparison, 3D fabrics with the same structure composed of commercial antimicrobial yarns containing Ag ion and control yarns of the same types are also fabricated. The disk diffusion test shows that the fabrics and the yarns with AgNPs nanofibers and Ag ions suppress the growth of bacterial colonies. A long term filtration performance test show that the fabric filter containing AgNPs has 40-50% higher fluxes and substantially larger flux recovery proportions than those of the corresponding control filter. The scanning electron microscopy and confocal laser scanning microscopy analyses show that the fabric filter with AgNPs nano fibers has the lowest bacterial cell, polysaccharides, and protein clusters on surface and inside the first two layers of the fabric filters. This is achieved with a concentration of antimicrobial agent two orders of magnitude lower than a regular commercial antimicrobial filter, showing the efficiency of using antimicrobial nanofibers in a fabric filter. (C) 2016 Elsevier B.V. All rights reserved.