The aim of this study was the preparation and characterization of solid lipid microparticles (SLMs) with liquid crystal structure. In order to observe the morphology of SLMs, environmental emission scanning electron microscopy was employed. And the structure of particles measured by polarized light optical microscopy and X-ray diffraction. Differential scanning calorimetry (DSC) was used to measure the crystallinity. Karl Fischer Titration were used to measure the micro amount of water content. The optimal formation was prepared. The influence of homogenization rate, emulsification temperature, concentration of NaCl, co-emulsifier type and concentration on particle size was discussed.