Ligth amplification properties are investigated for single crystals of commercially available 2,5-bis(4-biphenylyl)thiophene. Depending on growth methods, three kinds of crystals are obtained having different fluorescence spectra in blue-green color. Under optical pumping, their amplified spontaneous emission (ASE) bands appear at different wavelengths. ;The solution-grown crystal shows ASE bands at lambda = 464 and 494 nm which are assigned to the 0-1 and 0-2 transitions, respectively. By contrast, the vapor-and slide-boat-grown crystals show a red-shifted 0-1 band at lambda = 496 and a 0-2 band at 520 nm, respectively.