Intrinsic electrocatalytic activities of a material and electrode architecture for supporting the active material are crucial factors that determine the electrochemical sensing performance. In this work, tetragonal beta-MnO2 is taken as an electrocatalytic material for enzymeless glucose sensing. To construct a three dimensional nanostructure electrode, beta-MnO2 is directly grown on flexible carbon fiber fabric forming the micro/nanorod arrays (RA). For comparison, beta-MnO2 micro/nanorod powder (RP) is immobilized on graphite electrode by Nafion binder. Both beta-MnO2 RA and RP electrodes are investigated systematically as enzymeless glucose sensors and the results indicate the high sensitivity of 1650.6 mu AmM(-1)cm(-2), wide linear range up to 4.5 mM for the RA electrode, which shows much better performance than that of RA electrode. The sensitivity of RA electrode towards electro-oxidation of glucose is about 1.7-fold as much as that for RP electrode, mainly benefiting from the favorable electrode architecture. Besides, the RA electrode shows extraordinary stability, reproducibility and selectivity. Our investigation indicates that the electrode based on the beta-MnO2 micro/nanorod array directly grown on carbon fiber fabric is outstanding candidate for high efficiency flexible enzymeless glucose sensing. (C) 2016 Elsevier Ltd. All rights reserved.