This study focused on the incorporation of silver nanoparticles and chitosan at poly(hydroxybutyrate) (PHB) film surfaces by a two-step method: helium plasma followed by wet chemical treatments. Silver nanoparticles were obtained by a facile green synthesis in a chitosan solution, in which the plasma-treated PHB samples were immersed. The increase in plasma treatment times provided an increase at PHB surface of the silver nanoparticles content, which proved to have a coreshell shape and diameters between 14-16 nm.