Environmental standardization and stabilization of surface charges of silver nanoparticles (AgNPs) is important in biological systems and interest in bio-interfacial interaction. Different synthesized AgNPs in chemical reduced (AgNO3 (0.01, 0.1 and 0.5 M); NaBH4 and Na3C6H5O7) garnered for analysis of physico-chemical charge stabilization by means of different pH (1-13) and ionic interferences (NaCI, Ca(NO3)(2), Na2CO3 and NaNO3). The uniform sized (size: similar to 22 nm) and highly charged (zeta potential: -37.9 mV) AgNPs with uniform dispersion remains unaltered in high ionic interferences. Highest anti fungal activity of AgNPs against Candida albicans and moderate activity against Staphylococcus aureus are correlated. (C) 2016 Elsevier B.V. All rights reserved.