Application of Bacillus pumilus b-xylosidase reaction and simulated moving bed purification to efficient production of high-purity xylobiose from xylose

Chanhun Park; Jaehwan Choi; Myungok Kyung; Sheungwoo Seo; Sung-Eun Jo; Kyungsun Lee; Pungho Kim; Nien-Hwa Linda Wang; Sangwon Jung; Sungyong Mun

Journal of Industrial and Engineering Chemistry, Vol.47, 431-438, March, 2017

DOI10.1016/j.jiec.2016.12.015 Export Citation

Application of Bacillus pumilus b-xylosidase reaction and simulated moving bed purification to efficient production of high-purity xylobiose from xylose

Chanhun Park¹, Jaehwan Choi¹, Myungok Kyung^{2, 3}, Sheungwoo Seo^{2, 4}, Sung-Eun Jo^{2, 4}, Kyungsun Lee^{2, 4}, Pungho Kim¹, Nien-Hwa Linda Wang⁵, Sangwon Jung^{2, 4, †}, and Sungyong Mun^{1, †}

¹Department of Chemical Engineering, Hanyang University, Haengdang-dong, Seongdong-gu, Seoul, 04763, Republic of Korea
²R&D Center, TS Corporation, 116, Wolmi-ro, Jung-Ku, Incheon, 22300, Republic of Korea
³Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, 1, Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea
⁴, Korea
⁵School of Chemical Engineering, 480 Stadium Mall Drive, Purdue University, West Lafayette, IN 47907-2100, USA

E-mail:,

Xylobiose (X2) is recognized to possess great prebiotic function and to be highly favorable for application in food and prebiotic industries. In this study, we demonstrated that the cloned b-xylosidase of Bacillus pumilus IPO could be utilized to produce X2 via the reaction of xylose(X1)→xylobiose(X2). The use of such enzyme in the X1 → X2 reaction was found to give much higher X2 reaction yield and reaction efficiency, compared to those reported in the literature. Furthermore, we developed an efficient simulated moving bed (SMB) chromatographic process that could recover X2 from the reaction output with nearly 100% purity and 92% recovery on a continuous-separation mode. The developed SMB process could also recover the unreacted X1 almost completely, which leaves room for a further increase in the overall X2 reaction yield by reusing the recovered X1 from the SMB as the reactant of the upstream processing (i.e., B. pumilus IPO b-xylosidase X1 → X2 reaction). The results of this study will enable a highly economical and environmentally-friendly production of high-purity X2 from X1.

Keywords:Simulated moving bed;Xylobiose;Enzyme reaction;Separation;Purification

[References]

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Vazquez MJ, Alonso JL, Dominguez H, Parajo JC, Trends Food Sci. Technol., 11, 387 (2000)
Yuan QP, Zhang H, Qian ZM, Yang XJ, J. Chem. Technol. Biotechnol., 79(10), 1073 (2004)
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Chung SF, Wen CY, AIChE J., 14, 857 (1968)
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Wilke CR, Chang PIN, AIChE J., 1, 264 (1955)
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[2] Suwa Y, Koga K, Fujikawa S, Okazaki M, Irie T, Nakada T, Bifidobacterium bifidum proliferation promoting composition containing xylooligosaccharide, U.S Pat. 5939309, 1999.

[3] Vazquez MJ, Alonso JL, Dominguez H, Parajo JC, Trends Food Sci. Technol., 11, 387 (2000)

[4] Yuan QP, Zhang H, Qian ZM, Yang XJ, J. Chem. Technol. Biotechnol., 79(10), 1073 (2004)

[5] Okazaki M, Fujikawa S, Matsumoto N, J. Jpn. Soc. Nutr. Food Sci., 43, 395 (1990)

[6] Okazaki M, Fujikawa S, Matsumoto N, Bifidobact. Microflora, 9, 77 (1990)

[7] Howard MD, Gordon DT, Garleb KA, Kerley MS, J. Nutr., 125, 2604 (1995)

[8] Moura P, Barata R, Carvalheiro F, Girio F, Loureiro-Dias MC, Esteves MP, Lebensm. Wiss. Technol., 40, 963 (2007)

[9] Katapodis P, Nerinckx W, Claeyssens M, Christakopoulos P, Process Biochem., 41(12), 2402 (2006)

[10] Guerfali M, Gargouri A, Belghith H, Appl. Biochem. Biotechnol., 150(3), 267 (2008)

[11] Hur JS, Wankat PC, Ind. Eng. Chem. Res., 45(4), 1426 (2006)

[12] O'Brien AG, Horvath Z, Levesque F, Lee JW, Seidel-Morgenstern A, Seeberger PH, Angew. Chem.-Int. Edit., 51, 7028 (2012)

[13] Song SM, Kim IH, Korean J. Chem. Eng., 30(8), 1527 (2013)

[14] Lee JW, Horvath Z, O’Brien AG, Seeberger PH, Seidel-Morgenstern A, Chem. Eng. J., 25, 355 (2014)

[15] Moriyama H, Fukusaki E, Crespo JC, Shinmyo A, Okada H, Eur. J. Biochem., 166, 539 (1987)

[16] Xu WZ, Shima Y, Negoro S, Urabe I, Eur. J. Biochem., 202, 1197 (1991)

[17] Panbangred W, Kondo T, Negoro S, Shinmyo A, Kada H, Mol. Gen. Genet., 192, 335 (1983)

[18] Terpe K, Appl. Microbiol. Biotechnol., 72(2), 211 (2006)

[19] Wagschal K, Heng C, Lee CC, Robertson GH, Orts WJ, Wong DWS, Appl. Biochem. Biotechnol., 155(1-3), 304 (2009)

[20] Wisniewski L, Pereira CSM, Polakovic M, Rodrigues AE, Adsorption, 20, 483 (2014)

[21] Choi JH, Park H, Park C, Wang NHL, Mun S, J. Chromatogr. A, 1465, 143 (2016)

[22] Chin CY, Wang NHL, Sep. Purif. Rev., 33, 77 (2004)

[23] Wankat PC, Rate-Controlled Separations, Blackie Academic & Professional, New York, 1994.

[24] Chung SF, Wen CY, AIChE J., 14, 857 (1968)

[25] Wilson EJ, Geankoplis CJ, Ind. Eng. Chem. Fundam., 5, 9 (1966)

[26] Wilke CR, Chang PIN, AIChE J., 1, 264 (1955)

[27] Mackie JS, Meares P, Proc. R. Soc. Lond. Ser. A, 232, 498 (1955)

[28] Kasat RB, Gupta SK, Comput. Chem. Eng., 27(12), 1785 (2003)

[29] Lee KB, Kasat RB, Cox GB, Wang NHL, AIChE J., 54(11), 2852 (2008)

[30] Ma Z, Wang NH, AIChE J., 43(10), 2488 (1997)

[31] Kebler LC, Seidel-Morgenstern A, J. Chromatogr. A, 1207, 55 (2008)