화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.47, 336-347, March, 2017
DOI10.1016/j.jiec.2016.12.004  Export Citation
In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene
Marija Dosic1, Sanja Erakovic2, Ana Jankovic2, Maja Vukasinovuc-Sekulic2, Ivana Z. Matic3, Jovica Stojanovic1, Kyong Yop Rhee4, †, Vesna Miskovic-Stankovic2, 4, †, and Soo-Jin Park5, †
  • 1Institute for Technology of Nuclear and Other Mineral Raw Materials, Bulevar Frans d'Eperea 86, 11000 Belgrade, Serbia
  • 2Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11 000 Belgrade, Serbia
  • 3Institute of Oncology and Radiology of Serbia, Pasterova 14, 11 000 Belgrade, Serbia
  • 4Department of Mechanical Engineering, Kyung Hee University, Yongin 449-701 South Korea
  • 5Department of Chemistry, Inha University, Korea
E-mail:, ,
Graphene (Gr) and natural polymer chitosan (CS) were introduced to hydroxyapatite (HAP) to produce a three-component composite coating, which was fabricated by cathodic electrophoretic deposition on Ti substrates in an ethanol suspension. These HAP/CS/Gr coatings were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and electrochemical measurements and found that the graphene into HAP/CS composites significantly improves their morphology, thermal stability, and bioactivity. Both HAP/CS and HAP/CS/Gr composite coatings are classified as non-cytotoxic when tested against healthy peripheral blood mononuclear cells (PBMC), while antibacterial activity against Staphylococcus aureus and Escherichia coli could not be verified.
Keywords:Coating materials;Composite materials;Electrochemical impedance spectroscopy;X-ray diffraction;Scanning electron microscopy
  1. Gopi D, Nithiya S, Shinyjoy E, Kavitha L, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., doi:http://dx.doi.org/10.1016/j.saa.2012.02.069., 92, 194 (2012)
  2. Salinas AJ, Shruti S, Malavasi G, Menabue L, Vallet-Regi M, Acta Biomater., doi:http://dx.doi.org/10.1016/j.actbio.2011.05.033., 7(9), 3452 (2011)
  3. Looney M, O’shea H, Redington W, Kelly G, Boyd D, J. Non-Cryst. Solids, doi:http://dx.doi.org/10.1016/j.jnoncrysol.2010.11.118., 357, 2097 (2011)
  4. Gopi D, Indira J, Kavitha L, Sekar M, Mudali UK, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., doi:http://dx.doi.org/10.1016/j.saa.2012.02.033., 93, 131 (2012)
  5. Gopi D, Shinyjoy E, Sekar M, Surendiran M, Kavitha L, Kumar TSS, Corrosion Sci., doi:http://dx.doi.org/10.1016/j.corsci.2013.04.021., 73, 321 (2013)
  6. Jankovic A, Erakovic S, Vukasinovic-Sekulic M, Miskovic-Stankovic V, Park SJ, Rhee KY, Prog. Org. Coat., doi:http://dx.doi.org/10.1016/j.porgcoat.2015.01.019., 83, 1 (2015)
  7. Jankovic A, Erakovic S, Mitric M, Matic IZ, Juranic ZD, Tsui GCP, Tang CY, Miskovic-Stankovic V, Rhee KY, Park SJ, J. Alloy. Compd., doi:http://dx.doi.org/10.1016/j.jallcom.2014.11.078., 624, 148 (2015)
  8. Erakovic S, Jankovic A, Matic IZ, Juranic ZD, Vukasinovic-Sekulic M, Stevanovic T, Miskovic-Stankovic V, Mater. Chem. Phys., doi:http://dx.doi.org/10.1016/j.matchemphys.2013.07.047., 142(2-3), 521 (2013)
  9. Pramanik N, Mishra D, Banerjee I, Maiti TK, Bhargava P, Pramanik P, Int. J. Biomater., doi:http://dx.doi.org/10.1155/2009/512417., 2009, 512417 (2009)
  10. Tanase CE, Popa MI, Verestiuc L, Proceeding-2009 Advanced Technologies for Enhanced Quality of Life, AT-EQUAL, July, 2009, pp. 42, doi:http://dx.doi.org/10.1109/AT-EQUAL.2009.19.
  11. Kaya M, Seyyar O, Baran T, Turkes T, Front. Zool., doi:http://dx.doi.org/10.1186/s12983-014-0059-8., 11(1), 59 (2014)
  12. Zhitomirsky D, Roether JA, Boccaccini AR, Zhitomirsky I, J. Mater. Process. Technol., doi:http://dx.doi.org/10.1016/j.jmatprotec.2008.04.034., 209, 1853 (2009)
  13. Lahiji A, Sohrabi A, Hungerford DS, Frondoza CG, J. Biomed. Mater. Res., doi:http://dx.doi.org/10.1002/1097-4636(20000915)51., 51, 586 (2000)
  14. Kim IY, Seo SJ, Moon HS, Yoo MK, Park IY, Kim BC, Cho CS, Biotechnol. Adv., doi:http://dx.doi.org/10.1016/j.biotechadv.2007.07.009., 26, 1 (2008)
  15. Sayyar S, Murray E, Thompson BC, Chung J, Officer DL, Gambhir S, Spinks GM, Wallace GG, J. Mater. Chem., doi:http://dx.doi.org/10.1039/C4TB01636J., 3(3), 481 (2015)
  16. Yamaguchi I, Tokuchi K, Fukuzaki H, Koyama Y, Takakuda K, Monma H, Tanaka J, J. Biomed. Mater. Res., doi:http://dx.doi.org/10.1002/1097-4636(200104)55:1<20:AID-JBM30>3.0.CO;2-F., 55, 20 (2001)
  17. Shakir M, Jolly R, Khan MS, Iram NE, Sharma TK, Al-Resayes SI, Polym. Adv. Technol., doi:http://dx.doi.org/10.1002/pat.3417., 26(1), 41 (2015)
  18. Karakecili A, Arikan A, Plast. Res. Online (n.d.) 3. doi:http://dx.doi.org/10.1002/spepro.004334.
  19. Nikpour MR, Rabiee SM, Jahanshahi M, Compos. B Eng., doi:http://dx.doi.org/10.1016/j.compositesb.2012.01.056., 43(4), 1881 (2012)
  20. Sayyar S, Murray E, Gambhir S, Spinks G, Wallace GG, Officer DL, JOM, doi:http://dx.doi.org/10.1007/s11837-015-1549-7., 68(1), 384 (2016)
  21. Shen H, Zhang L, Liu M, Zhang Z, Theranostics, doi:http://dx.doi.org/10.7150/thno.3642., 2(3), 283 (2012)
  22. Zhang L, Liu W, Yue C, Zhang T, Li P, Xing Z, Chen Y, Carbon, doi:http://dx.doi.org/10.1016/j.carbon.2013.04.074., 61, 105 (2013)
  23. Kim S, Ku SH, Lim SY, Kim JH, Park CB, Adv. Mater., doi:http://dx.doi.org/10.1002/adma.201100010., 23(17), 2009 (2011)
  24. Chavez-Valdez A, Shaffer MSP, Boccaccini AR, J. Phys. Chem., doi:http://dx.doi.org/10.1021/jp3064917., 117(6), 1502 (2013)
  25. Surudzic R, Jankovic A, Bibic N, Vukasinovic-Sekulic M, Peric-Grujic A, Miskovic-Stankovic V, Park SJ, Rhee KY, Compos. B Eng., doi:http://dx.doi.org/10.1016/j.compositesb.2015.09.029., 85, 102 (2016)
  26. Neelgund GM, Oki A, Luo ZP, Mater. Res. Bull., doi:http://dx.doi.org/10.1016/j.materresbull.2012.08.077., 48(2), 175 (2013)
  27. Zhang C, Liu T, Chin. Sci. Bull., doi:http://dx.doi.org/10.1007/s11434-012-5321-x., 57(23), 3010 (2012)
  28. Santos CM, Mangadlao J, Ahmed F, Leon A, Advincula RC, Rodrigues DF, Nanotechnology, doi:http://dx.doi.org/10.1088/0957-4484/23/39/395101., 23, 395101 (2012)
  29. Fan HL, Wang LL, Zhao KK, Li N, Shi ZJ, Ge ZG, Jin ZX, Biomacromolecules, doi:http://dx.doi.org/10.1021/bm100470q., 11(9), 2345 (2010)
  30. Shi Y, Li M, Liu Q, Jia Z, Xu X, Cheng Y, Zheng Y, J. Mater. Sci. -Mater. Med., doi:http://dx.doi.org/10.1007/s10856-015-5634-9., 27, 48 (2016)
  31. Geetha V, Gomathi T, Sudha PN, J. Chem. Pharm. Res., 7(5), 871 (2015)
  32. Miskovic-Stankovic V, Erakovic S, Djosic M, Jankovic A, Pat. Appl. No. P-2015/0785, Intellect. Prop. Off. Repub. Serbia, Dated 25.11.2015 (2015) 2015.
  33. Liu C, Zhang J, Yifeng E, Yue J, Chen L, Li D, Electron. J. Biotechnol., doi:http://dx.doi.org/10.1016/j.ejbt.2014.04.013., 17(4), 183 (2014)
  34. Li M, Wang Y, Liu Q, Li Q, Cheng Y, Zheng Y, Xi T, Wei S, J. Mater. Chem. B, doi:http://dx.doi.org/10.1039/C2TB00053A., 1(4), 475 (2013)
  35. Bustos-Ramirez K, Martinez-Hernandez AL, Martinez-Barrera G, de Icaza M, Castano VM, Velasco-Santos C, Materials (Basel), doi:http://dx.doi.org/10.3390/ma6030911., 6(3), 911 (2013)
  36. Tadic D, Epple M, Biomaterials, doi:http://dx.doi.org/10.1016/S0142-9612(03)00621-5., 25(6), 987 (2004)
  37. Djosic MS, Mitric M, Miskovic-Stankovic VB, J. Serb. Chem. Soc., doi:http://dx.doi.org/10.2298/JSC140626098D., 80, 237 (2015)
  38. Venkatesan J, Kim SK, Mar. Drugs, doi:http://dx.doi.org/10.3390/md8082252., 8(8), 2252 (2010)
  39. Depan D, Pesacreta TC, Misra RDK, Biomater. Sci., doi:http://dx.doi.org/10.1039/c3bm60192g., 2(2), 264 (2014)
  40. Szatkowski T, Kolodziejczak-Radzimska A, Zdarta J, Szwarc-Rzepka K, Paukszta D, Wysokowski M, Ehrlich H, Jesionowski T, Physicochem. Prob. Miner. Process., doi:http://dx.doi.org/10.1002/jbm.10280., 51(2), 575 (2015)
  41. Lopez FA, Merce ALR, Alguacil FJ, Lopez-Delgado A, J. Therm. Anal. Calorim., 91(2), 633 (2008)
  42. Wanjun T, Cunxin W, Donghua C, Polym. Degrad. Stabil., doi:http://dx.doi.org/10.1016/j.polymdegradstab.2004.08.006., 87(3), 389 (2005)
  43. Cordero-Arias L, Cabanas-Polo S, Gao H, Gilabert J, Sanchez E, Roether JA, Schubert DW, Virtanene S, Boccaccini AR, RSC Adv., doi:http://dx.doi.org/10.1039/c3ra40535d., 3, 11247 (2013)
  44. Lawrie G, Keen I, Drew B, Chandler-Temple A, Rintoul L, Fredericks P, Grondahl L, Biomacromolecules, 8(8), 2533 (2007)
  45. Tang S, Tian B, Guo YJ, Zhu ZA, Guo YP, Surf. Coat. Technol., doi:http://dx.doi.org/10.1016/j.surfcoat.2014.04.028., 251, 210 (2014)
  46. Batmanghelich F, Ghorbani M, Ceram. Int., doi:http://dx.doi.org/10.1016/j.ceramint.2012.12.046., 39(5), 5393 (2013)
  47. Gebhardt F, Seuss S, Turhan MC, Hornberger H, Virtanen S, Boccaccini AR, Mater. Lett., doi:http://dx.doi.org/10.1016/j.matlet.2011.08.088., 66(1), 302 (2012)
  48. Rehman I, Bonfield W, J. Mater. Sci. -Mater. Med., doi:http://dx.doi.org/10.1023/A:1018570213546., 8(1), 1 (1997)
  49. Miskovic-Stankovic V, Erakovic S, Jankovic A, Vukasinovic-Sekulic M, Mitric M, Jung YC, Park SJ, Rhee KY, Carbon Lett., doi:http://dx.doi.org/10.5714/CL.2015.16.4.233., 16(4), 233 (2015)
  50. Reyes-Gasga J, Martinez-Pineiro EL, Rodriguez-Alvarez G, Tiznado-Orozco GE, Garcia-Garcia R, Bres EF, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., doi:http://dx.doi.org/10.1016/j.msec.2013.07.014., 33(8), 4568 (2013)
  51. Lian PC, Zhu XF, Liang SZ, Li Z, Yang WS, Wang HH, Electrochim. Acta, doi:http://dx.doi.org/10.1016/j.electacta.2010.02.025., 55(12), 3909 (2010)
  52. Zawadzki J, Kaczmarek H, Carbohydr. Polym., doi:http://dx.doi.org/10.1016/j.carbpol.2009.11.037., 80(2), 395 (2010)
  53. Granados-Correa F, Rev. Int., 26(2), 129 (2010)
  54. Capanema NS, Mansur AAP, Carvalho SM, Silva ARP, Ciminelli VS, Mansur HS, Materials (Basel), doi:http://dx.doi.org/10.3390/ma8074191., 8(7), 4191 (2015)
  55. Li M, Liu Q, Jia ZJ, Xu XC, Shi YY, Cheng Y, Zheng YF, Xi TF, Wei SC, Appl. Surf. Sci., doi:http://dx.doi.org/10.1016/j.apsusc.2013.08.012., 284, 804 (2013)
  56. Victoria EC, Gnanam FD, Trends Biomater. Artif. Organs, 16(1), 12 (2002)
  57. Roguska A, Pisarek M, Andrzejczuk M, Dolata M, Lewandowska M, Janik-Czachor M, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., doi:http://dx.doi.org/10.1016/j.msec.2011.02.009., 31(5), 906 (2011)
  58. Yao ZQ, Ivanisenko Y, Diemant T, Caron A, Chuvilin A, Jiang JZ, Valiev RZ, Qi M, Fecht HJ, Acta Biomater., doi:http://dx.doi.org/10.1016/j.actbio.2009.12.053., 6(7), 2816 (2010)
  59. Watling KM, Parr JF, Rintoul L, Brown CL, Sullivan LA, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., doi:http://dx.doi.org/10.1016/j.saa.2011.03.002., 80(1), 106 (2011)
  60. Sjogren G, Sletten G, Dahl JE, J. Prosthet. Dent., doi:http://dx.doi.org/10.1067/mpr.2000.107227., 84(2), 229 (2000)
  61. Orazem ME, Tribollet B, Electrochemical Impedance Spectroscopy, John Wiley Sons Inc., Hoboken, New Jersey, 2008, doi:http://dx.doi.org/10.1002/0471716243.
  62. Zhang Q, Chen J, Feng J, Cao Y, Deng C, Zhang X, Biomaterials, doi:http://dx.doi.org/10.1016/S0142-9612(03)00371-5., 24(26), 4741 (2003)
  63. Barrere F, van der Valk CM, Dalmeijer RAJ, van Blitterswijk CA, de Groot K, Layrolle P, J. Biomed. Mater. Res., doi:http://dx.doi.org/10.1002/jbm.a.10291., 64, 378 (2003)