Fabrication and characterization of 3D-printed bone-like β-tricalcium phosphate/polycaprolactone scaffolds for dental tissue engineering

JiSun Park; Sang Jin Lee; Ha Hyeon Jo; Jun Hee Lee; Wan Doo Kim; Jae Young Lee; Su A Park

Journal of Industrial and Engineering Chemistry, Vol.46, 175-181, February, 2017

DOI10.1016/j.jiec.2016.10.028 Export Citation

Fabrication and characterization of 3D-printed bone-like β-tricalcium phosphate/polycaprolactone scaffolds for dental tissue engineering

JiSun Park^{1, 2}, Sang Jin Lee^{1, 3}, Ha Hyeon Jo^{1, 3}, Jun Hee Lee^{1, 3}, Wan Doo Kim^{1, 3}, Jae Young Lee², and Su A Park^{1, 3, †}

¹Department of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and Materials (KIMM), 156 Gajeongbuk-ro, Yuseong-gu, Daejeon 304-343, Republic of Korea
²School of Material Science & Engineering, Gwangju Institute of Science and Technology (GIST), 123 Cheomdan Gwagi-ro, Buk-gu Gwangju 500-712, Republic of Korea
³, Korea

E-mail:

β-tricalcium phosphate (β-TCP) and polycaprolactone (PCL) composites were manufactured using the lab-made 3D bioprinting system to produce 50TCP50PCL (50% β-TCP with 50% PCL) and 70TCP30PCL (70% β-TCP with 30% PCL) composite scaffolds for bone tissue engineering. The 70TCP30PCL scaffold containing the highest β-TCP content exhibited rougher morphologies and more porous than the other scaffolds (i.e., PCL and 50TCP50PCL). In vitro studies revealed that cell proliferation and alkaline phosphate activity were improved on the β-TCP-based composite scaffolds. Our results suggest that our 3-D printed β-TCP-containing PCL scaffolds would benefit new dental applications or regeneration therapies.

Keywords:3D printing;β-TCP;Dental tissue regeneration;Scaffolds

[References]

Bose S, Roy M, Bandyopadhyay A, Trends Biotechnol., 30, 546 (2012)
Tamimi F, Torres J, Abedalla KA, Cabarcos EL, Alkhraisat MH, Bassett DC, Gbureck U, Barralet JE, Biomaterials, 35, 5436 (2014)
Levin L, J. Appl. Oral Sci., 16, 171 (2008)
Lee SJ, Lee DH, Yoon TR, Kim HK, Jo HH, Park JS, Lee JH, Kim WD, Kwon IK, Park SA, Acta Biomater., 40, 182 (2016)
Wang MO, Vorwald CE, Dreher ML, Mott EJ, Cheng MH, Cinar A, Mehdizadeh H, Somo S, Dean D, Brey EM, Fisher JP, Adv. Mater., 27(1), 138 (2015)
Zeltinger J, Sherwood JK, Graham DA, Mueller R, Griffith LG, Tissue Eng., 7, 557 (2001)
Wu C, Luo Y, Cuniberti G, Xiao Y, Gelinsky M, Acta Biomater., 7, 2644 (2011)
Karageorgiou V, Kaplan D, Biomaterials, 26, 5474 (2005)
Fedorovich NE, Kuipers E, Gawlitta D, Dhert WJA, Alblas J, Tissue Eng. A, 17, 2473 (2011)
Kothapalli CR, Shaw MT, Wei M, Acta Biomater., 1, 653 (2005)
Lee JB, Jeong SI, Bae MS, Yang DH, Heo DN, Kim CH, Alsberg E, Kwon IK, Tissue Eng. A, 17, 2695 (2011)
Tan KH, Chua CK, Leong KF, Cheah CM, Gui WS, Tan WS, Wiria FE, BioMedical Mater. Eng., 15, 113 (2005)
Guo H, Su J, Wei J, Kong H, Liu C, Acta Biomater., 5, 268 (2009)
Murphy SV, Atala A, Nat. Biotechnol., 32, 773 (2014)
Lee SJ, Heo DN, Park JS, Kwon SK, Lee JH, Kim WD, Kwon IK, Park SA, Phys. Chem. Chem. Phys., 17, 2996 (2015)
Sobral JM, Caridade SG, Sousa RA, Mano JF, Reis RL, Acta Biomater., 7, 1009 (2011)
Amirkhani S, Bagheri R, Yazdi AZ, Acta Mater., 60, 2778 (2012)
Hollister SJ, Nat. Mater., 4(7), 518 (2005)
Pati F, Song TH, Rijal G, Jang J, Kim SW, Cho DW, Biomaterials, 37, 230 (2015)
Wang X, Yan Y, Zhang R, Trends Biotechnol., 25, 505 (2007)
Bose S, Vahabzadeh S, Bandyopadhyay A, Mater. Today, 16, 496 (2013)
Seol YJ, Kang TY, Cho DW, Soft Matter, 8, 1730 (2012)
Williams DF, Biomaterials, 30, 5897 (2009)
Bohnel M, Mater. Today, 13, 24 (2010)
Park SA, Lee JB, Kim YE, Kim JE, Lee JH, Shin JW, Kwon IK, Kim WD, Macromol. Res., 22(8), 882 (2014)
Shao HJ, Lee YT, Chen CS, Wang JH, Young TH, Biomaterials, 31, 4695 (2010)
Temple JP, Hutton DL, Hung BP, Huri PY, Cook CA, Kondragunta R, Jia X, Grayson WL, J. Biomed. Mater. Res. A, 102, 4317 (2014)
Sun H, Mei L, Song C, Cui X, Wang P, Biomaterials, 27, 1735 (2006)
Kim JY, Cho DW, Micro Eng., 86, 1447 (2009)
Declercq HA, Desmet T, Berneel EE, Dubruel P, Cornelissen MJ, Acta Biomater., 9, 7699 (2013)
Jonas J, Evar KDC, Helle L, Bindzus FC, Muwan C, Albæk KA, Duedal RJH, Eric BC, Tissue Eng. A, 21, 729 (2015)
Shim JH, Huh JB, Park JY, Jeon YC, Kang SS, Kim JY, Rhie JW, Cho DW, Tissue Eng. A, 19, 317 (2012)
Xue W, Bandyopadhyay A, Bose S, J. Biomed. Mater. Res. B, 91B, 831 (2009)
Yeo A, Wong WJ, Khoo HH, Teoh SH, J. Biomed. Mater. Res. A, 92A, 311 (2010)
Luo Y, Zhai D, Huan Z, Zhu H, Xia L, Chang J, Wu C, ACS Appl. Mater. Interfaces, 7, 24377 (2015)
Xu M, Zhai D, Chang J, Wu C, Acta Biomater., 10, 463 (2014)
Zhou Y, Wu C, Xiao Y, Acta Biomater., 8, 2307 (2012)
Shim JH, Moon TS, Yun MJ, Jeon YC, Jeong CM, Cho DW, Huh JB, J. Mater. Sci. -Mater. Med., 23, 2993 (2012)
Kang HW, Lee SJ, Ko IK, Kengla C, Yoo JJ, Atala A, Nat. Biotechnol., 34, 312 (2016)
Galea L, Bohner M, Thuering J, Doebelin N, Aneziris CG, Graule T, Biomaterials, 34, 6388 (2013)
Xu HHK, Simon CG, Biomaterials, 26, 1337 (2005)
Fielding GA, Bandyopadhyay A, Bose S, Dent. Mater., 28, 113 (2012)
Xu HHK, Quinn JB, Takagi S, Biomaterials, 25, 1029 (2004)
Mondrinos MJ, Dembzynski R, Lu L, Byrapogub VKC, Wootton DM, Lelkes PI, Zhou J, Biomaterials, 27, 4399 (2006)
Yeo M, Lee H, Kim G, Biomacromolecules, 12(2), 502 (2011)
Macchetta A, Turner IG, Bowen CR, Acta Biomater., 5, 1319 (2009)
Giuliani A, Manescu A, Larsson E, Tromba G, Luongo G, Piattelli A, Mangano F, Iezzi G, Mangano C, Clin. Implant Dent. Relat. Res., 16, 736 (2014)
Felekori MN, Mesgar ASM, Mohammadi Z, Ceram. Int., 41, 6013 (2015)
Rutz AL, Hyland KE, Jakus AE, Burghardt WR, Shah RN, Adv. Mater., 27(9), 1607 (2015)
Teixeira S, Fernandes H, Leusink A, van Blitterswijk C, Ferraz MP, Monteiro FJ, de Boer J, J. Biomed. Mater. Res. A, 93A, 567 (2010)
Converse GL, Conrad TL, Roeder RK, J. Mech. Behav. Biomed. Mater., 2, 627 (2009)
Rosa V, Bona AD, Cavalcanti BH, Nor JE, Dent. Mater., 28, 341 (2012)
Wilt FH, Dev. Biol., 280, 15 (2005)

[1] Bose S, Roy M, Bandyopadhyay A, Trends Biotechnol., 30, 546 (2012)

[2] Tamimi F, Torres J, Abedalla KA, Cabarcos EL, Alkhraisat MH, Bassett DC, Gbureck U, Barralet JE, Biomaterials, 35, 5436 (2014)

[3] Levin L, J. Appl. Oral Sci., 16, 171 (2008)

[4] Lee SJ, Lee DH, Yoon TR, Kim HK, Jo HH, Park JS, Lee JH, Kim WD, Kwon IK, Park SA, Acta Biomater., 40, 182 (2016)

[5] Wang MO, Vorwald CE, Dreher ML, Mott EJ, Cheng MH, Cinar A, Mehdizadeh H, Somo S, Dean D, Brey EM, Fisher JP, Adv. Mater., 27(1), 138 (2015)

[6] Zeltinger J, Sherwood JK, Graham DA, Mueller R, Griffith LG, Tissue Eng., 7, 557 (2001)

[7] Wu C, Luo Y, Cuniberti G, Xiao Y, Gelinsky M, Acta Biomater., 7, 2644 (2011)

[8] Karageorgiou V, Kaplan D, Biomaterials, 26, 5474 (2005)

[9] Fedorovich NE, Kuipers E, Gawlitta D, Dhert WJA, Alblas J, Tissue Eng. A, 17, 2473 (2011)

[10] Kothapalli CR, Shaw MT, Wei M, Acta Biomater., 1, 653 (2005)

[11] Lee JB, Jeong SI, Bae MS, Yang DH, Heo DN, Kim CH, Alsberg E, Kwon IK, Tissue Eng. A, 17, 2695 (2011)

[12] Tan KH, Chua CK, Leong KF, Cheah CM, Gui WS, Tan WS, Wiria FE, BioMedical Mater. Eng., 15, 113 (2005)

[13] Guo H, Su J, Wei J, Kong H, Liu C, Acta Biomater., 5, 268 (2009)

[14] Murphy SV, Atala A, Nat. Biotechnol., 32, 773 (2014)

[15] Lee SJ, Heo DN, Park JS, Kwon SK, Lee JH, Kim WD, Kwon IK, Park SA, Phys. Chem. Chem. Phys., 17, 2996 (2015)

[16] Sobral JM, Caridade SG, Sousa RA, Mano JF, Reis RL, Acta Biomater., 7, 1009 (2011)

[17] Amirkhani S, Bagheri R, Yazdi AZ, Acta Mater., 60, 2778 (2012)

[18] Hollister SJ, Nat. Mater., 4(7), 518 (2005)

[19] Pati F, Song TH, Rijal G, Jang J, Kim SW, Cho DW, Biomaterials, 37, 230 (2015)

[20] Wang X, Yan Y, Zhang R, Trends Biotechnol., 25, 505 (2007)

[21] Bose S, Vahabzadeh S, Bandyopadhyay A, Mater. Today, 16, 496 (2013)

[22] Seol YJ, Kang TY, Cho DW, Soft Matter, 8, 1730 (2012)

[23] Williams DF, Biomaterials, 30, 5897 (2009)

[24] Bohnel M, Mater. Today, 13, 24 (2010)

[25] Park SA, Lee JB, Kim YE, Kim JE, Lee JH, Shin JW, Kwon IK, Kim WD, Macromol. Res., 22(8), 882 (2014)

[26] Shao HJ, Lee YT, Chen CS, Wang JH, Young TH, Biomaterials, 31, 4695 (2010)

[27] Temple JP, Hutton DL, Hung BP, Huri PY, Cook CA, Kondragunta R, Jia X, Grayson WL, J. Biomed. Mater. Res. A, 102, 4317 (2014)

[28] Sun H, Mei L, Song C, Cui X, Wang P, Biomaterials, 27, 1735 (2006)

[29] Kim JY, Cho DW, Micro Eng., 86, 1447 (2009)

[30] Declercq HA, Desmet T, Berneel EE, Dubruel P, Cornelissen MJ, Acta Biomater., 9, 7699 (2013)

[31] Jonas J, Evar KDC, Helle L, Bindzus FC, Muwan C, Albæk KA, Duedal RJH, Eric BC, Tissue Eng. A, 21, 729 (2015)

[32] Shim JH, Huh JB, Park JY, Jeon YC, Kang SS, Kim JY, Rhie JW, Cho DW, Tissue Eng. A, 19, 317 (2012)

[33] Xue W, Bandyopadhyay A, Bose S, J. Biomed. Mater. Res. B, 91B, 831 (2009)

[34] Yeo A, Wong WJ, Khoo HH, Teoh SH, J. Biomed. Mater. Res. A, 92A, 311 (2010)

[35] Luo Y, Zhai D, Huan Z, Zhu H, Xia L, Chang J, Wu C, ACS Appl. Mater. Interfaces, 7, 24377 (2015)

[36] Xu M, Zhai D, Chang J, Wu C, Acta Biomater., 10, 463 (2014)

[37] Zhou Y, Wu C, Xiao Y, Acta Biomater., 8, 2307 (2012)

[38] Shim JH, Moon TS, Yun MJ, Jeon YC, Jeong CM, Cho DW, Huh JB, J. Mater. Sci. -Mater. Med., 23, 2993 (2012)

[39] Kang HW, Lee SJ, Ko IK, Kengla C, Yoo JJ, Atala A, Nat. Biotechnol., 34, 312 (2016)

[40] Galea L, Bohner M, Thuering J, Doebelin N, Aneziris CG, Graule T, Biomaterials, 34, 6388 (2013)

[41] Xu HHK, Simon CG, Biomaterials, 26, 1337 (2005)

[42] Fielding GA, Bandyopadhyay A, Bose S, Dent. Mater., 28, 113 (2012)

[43] Xu HHK, Quinn JB, Takagi S, Biomaterials, 25, 1029 (2004)

[44] Mondrinos MJ, Dembzynski R, Lu L, Byrapogub VKC, Wootton DM, Lelkes PI, Zhou J, Biomaterials, 27, 4399 (2006)

[45] Yeo M, Lee H, Kim G, Biomacromolecules, 12(2), 502 (2011)

[46] Macchetta A, Turner IG, Bowen CR, Acta Biomater., 5, 1319 (2009)

[47] Giuliani A, Manescu A, Larsson E, Tromba G, Luongo G, Piattelli A, Mangano F, Iezzi G, Mangano C, Clin. Implant Dent. Relat. Res., 16, 736 (2014)

[48] Felekori MN, Mesgar ASM, Mohammadi Z, Ceram. Int., 41, 6013 (2015)

[49] Rutz AL, Hyland KE, Jakus AE, Burghardt WR, Shah RN, Adv. Mater., 27(9), 1607 (2015)

[50] Teixeira S, Fernandes H, Leusink A, van Blitterswijk C, Ferraz MP, Monteiro FJ, de Boer J, J. Biomed. Mater. Res. A, 93A, 567 (2010)

[51] Converse GL, Conrad TL, Roeder RK, J. Mech. Behav. Biomed. Mater., 2, 627 (2009)

[52] Rosa V, Bona AD, Cavalcanti BH, Nor JE, Dent. Mater., 28, 341 (2012)

[53] Wilt FH, Dev. Biol., 280, 15 (2005)