미세액적 광생물반응기를 활용한 광독립영양배양에서 Chlamydomonas reinhardtii의 성장성 분석

성영준; 곽호석; 최홍일; 김영환; 심상준; Young Joon Sung; Ho Seok Kwak; Hong Il Choi; Jaoon Young Hwan Kim; Sang Jun Sim

Korean Chemical Engineering Research, Vol.55, No.1, 80-85, February, 2017

DOI10.9713/kcer.2017.55.1.80 Export Citation

미세액적 광생물반응기를 활용한 광독립영양배양에서 Chlamydomonas reinhardtii의 성장성 분석

Growth Analysis of Chlamydomonas reinhardtii in Photoautotrophic Culture with Microdroplet Photobioreactor System

성영준¹, 곽호석¹, 최홍일¹, 김영환¹, 심상준^{1, 2, †}

¹고려대학교 화공생명공학과, 02841 서울특별시 성북구 안암로 145
²고려대학교 그린스쿨, 02841 서울특별시 성북구 안암로 145

Young Joon Sung¹, Ho Seok Kwak¹, Hong Il Choi¹, Jaoon Young Hwan Kim¹, and Sang Jun Sim^{1, 2, †}

¹Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul, 02841, Korea
²Green School, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul, 02841, Korea

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초록

최근 고부가가치 산물의 생산이 가능한 미세조류는 이산화탄소의 생물학적 전환 측면에서 많은 주목을 받고 있다. 그렇지만 미세조류 종 자체가 지닌 낮은 광합성 효율 및 생산성의 한계는 미세조류를 활용한 공정의 상업화를 막는 장애요인이다. 따라서 본 연구에서는 대표 미세조류 Chlamydomonas reinhardtii의 광독립영양 성장성 분석을 위한 미세액적 광생물반응기를 개발하였다. PDMS 기반의 미세유체 칩 내에 미세기둥을 배열하고 미세챔버의 높이를 조절하여 미세액적 내 이산화탄소의 전달속도를 증가시켰으며, 이는 세포 성장성과 형광 세기 변화를 통해 확인하였다. 마지막으로 미세액적 광생물반응기를 활용하여 다양한 이산화탄소 농도 및 광량 조건에서 C. reinhardtii의 광독립영양배양에서 성장성을 96 시간동안 관찰하고 분석하였다. 본 연구 결과를 통해 미세액적 광생물반응기는 성장성 및 유용물질 생산성이 우수한 미세조류 종을 빠르게 분석하고 쉽게 분리할 수 있는 효율적인 플랫폼임을 입증하였다.

Recently, microalgae which can produce high-value products have attracted increasing attention for biological conversion of CO2. However, low photosynthetic efficiency and productivity have limited the practical use of microalgae. Thus, we developed microdroplet photobioreactor for the analysis of photoautotrophic growth of model alga, Chlamydomonas reinhardtii. CO2 transfer rate was increased by integrating micropillar arrays and adjusting height of microchamber. These results were identified by change of cell growth rate and fluorescence intensity. Lastly, the photoautotrophic growth kinetics of C. reinhardtii in microdroplet photobioreactor were investigated under different CO2 concentrations and light intensities for 96 hours. As a result, microdroplet photobioreactor was efficient platform for isolation and rapid evaluation of microalgal strains which have enhanced productivity of high-value products and growth performance.

Keywords:Microdroplet;Photobioreactor;Chlamydomonas reinhardtii;Photoautotrophic Culture;CO2 Permeation

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Vonshak A, Torzillo G, Tomaseli L, J. Appl. Phycol., 6(1), 31 (1994)

[Referenced By]

[1] Chisti Y, Trends Biotechnol., 26(3), 126 (2008)

[2] Christenson L, Sims R, Biotechnol. Adv., 29(6), 686 (2011)

[3] Mata TM, Martins AA, Caetano NS, Renew. Sust. Energ. Rev., 14(1), 217 (2010)

[4] Pauly M, Keegstra K, Plant J., 54(4), 559 (2008)

[5] Pienkos PT, Darzins A, Biofuels, Bioprod. Biorefin., 3(4), 431 (2009)

[6] Stephens E, Ross IL, King Z, Mussgnug JH, Kruse O, Posten C, Borowitzka MA, Hankamer B, Nat. Biotechnol., 28(2), 126 (2010)

[7] Weyer KM, Bush DR, Darzins A, Willson BD, Bioenergy Res., 3(2), 204 (2010)

[8] Park JY, Lee GA, Kim KY, Kim KY, Choi SA, Jeong MJ, Oh YK, Korean Chem. Eng. Res., 52(1), 88 (2014)

[9] Lim HS, Kim JYH, Kwak HS, Sim SJ, Anal. Chem., 86(17), 8585 (2014)

[10] Dewan A, Kim J, McLean RH, Vanapalli SA, Karim MN, Biotechnol. Bioeng., 109(12), 2987 (2012)

[11] Fair RB, Microfluid. Nanofluid., 3(3), 245 (2007)

[12] Link DR, Grasland-Mongrain E, Duri A, Sarrazin F, Cheng Z, Cristobal G, Marquez M, Weitz DA, Angew. Chem.-Int. Edit., 45(16), 2556 (2006)

[13] Schneider T, Kreutz J, Chiu DT, Anal. Chem., 85(7), 3476 (2013)

[14] Pan J, Stephenson AL, Kazamia E, Huck WT, Dennis JS, Smith AG, Abell C, Integr. Biol., 3(10), 1043 (2011)

[15] Chen F, Trends Biotechnol., 14(11), 421 (1996)

[16] Morales-Sanchez D, Tinoco-Valencia R, Kyndt J, Martinez A, Biotechnol. Biofuels, 6(100), 1 (2013)

[17] Mata A, Fleischman A, Roy S, Biomed. Microdevices, 7(4), 281 (2005)

[18] van Poll ML, Zhou F, Ramstedt M, Hu L, Huck WTS, Angew. Chem.-Int. Edit., 46(35), 6634 (2007)

[19] Zhou JW, Ellis AV, Voelcker NH, Electrophoresis, 31(1), 2 (2010)

[20] Whitesides GM, Ostuni E, Takayama S, Jiang X, Ingber DE, Annu. Rev. Biomed. Eng., 3(1), 335 (2001)

[21] Chu CS, Lo YL, Sens. Actuators B-Chem., 129(1), 120 (2008)

[22] Grima EM, Sevilla JF, Perez JS, Camacho FG, J. Biotechnol., 45(1), 59 (1996)

[23] Vonshak A, Torzillo G, Tomaseli L, J. Appl. Phycol., 6(1), 31 (1994)