Wood, bark and leaves of Populus deltoides x maximowiczii and Populus trichocarpa were hydrolyzed with H2SO4, (72%) to monosaccharides to compare the profile of sugars. The wood was the best raw material to EtOH prodn., and the leaves were the worst one. The xylose and glucose content in hydrolyzates of wood of Populus deltoides x maximowiczii were 15.6% and 48.6%, while the contents of sugars from wood of Populus trichocarpa were 18.2% and 51.3%. Regardless of the studied biomass type, the contents of monosaccharides from Populus trichocarpa were higher than that from Populus deltoides x maximowiczii. A fairly high glucose content (31.1%) in hydrolyzate of the Populus trichocarpa bark was obsd.