화학공학소재연구정보센터
Journal of Structural Biology, Vol.196, No.3, 407-413, 2016
Iterative projection algorithms for ab initio phasing in virus crystallography
Iterative projection algorithms are proposed as a tool for ab initio phasing in virus crystallography. The good global convergence properties of these algorithms, coupled with the spherical shape and high structural redundancy of icosahedral viruses, allows high resolution phases to be determined with no initial phase information. This approach is demonstrated by determining the electron density of a virus crystal with 5-fold non-crystallographic symmetry, starting with only a spherical shell envelope. The electron density obtained is sufficiently accurate for model building. The results indicate that iterative projection algorithms should be routinely applicable in virus crystallography, without the need for ancillary phase information. (C) 2016 Elsevier Inc. All rights reserved.