A Prussian blue (PB)-gold nanocomposite ((PB-Au)(x), where x denotes the number of PB-Au bilayer) is prepared, by step-by-step electrodeposition of PB and gold on a glassy carbon electrode (GCE). The optimized (PB-Au)(2)/GCE exhibits excellent electrocatalytic activity for electroreduction of H2O2, and the cathodic current at -0.05 V responds linearly to H2O2 concentration up to 3.88 mM with a sensitivity of 1.32 mA mM(-1) cm(-2), which outperforms the gold-PB nanocomposites electrodeposited through other reported protocols. Moreover, a glucose oxidase (GOx)-polydopamine (PDA)-Pt nanoparticles (PtNPs) bionanocomposite is synthesized through chemical oxidation of dopamine by sodium chloroplatinate in the presence of GOx. A chitosan (CS)/GOx-PDA-PtNPs/(PB-Au)(2)/GCE is fabricated by casting GOx-PDA-PtNPs and then CS on (PB-Au)(2)/GCE. The cathodic current at 0.05 V on this enzyme electrode responds linearly to glucose concentration from 5 mu M to 0.79 mM with a sensitivity of 114 mu A mM(-1) cm(-2) and a LOD of 0.5 mu M (S/N = 3). The presented protocol can flexibly balance the high catalytic activity and the poor electron-conductance of PB for improving the electroactivity and electrocatalytic activity of PB-based films, thus yielding high-performance PB-based electrodes for low-potential determination of H2O2 and glucose. (C) 2016 Elsevier B.V. All rights reserved.