This study investigated the effects of pretreatment methods (such as autoclave, ultra-sonication and electrolysis) of mixed microalgae consortia (predominantly composed of Scenedesmus followed by Chlorella species) from natural ecological niche. In addition, the cultivated biomass (wet) was subsequently utilized for fermentative H-2 production in mesophilic regime. The results showed that peak hydrogen production rate (HPR) and hydrogen yield (HY) were achieved from electrolysis pretreated algal consortia as 236 +/- 14 mL/L-d and 37.7 +/- 0.4 mL/g (volatile solids) VSadded, whereas the untreated algal consortia resulted in the turnout as 64 +/- 5 mL/L-d and 9.5 +/- 0.0 mL/g VSadded, respectively. The significant increment observed in HPR and HY values were nearly 4 times higher. The solubilization of organic matter during the pretreatment showed positive correlation with the H-2 production. The energy generation rate and yield of the corresponding pretreatment methods were as follows, 1.44, 1.79 and 2.65 kJ/L-d for autoclave, ultra-sonication and electrolysis, the corresponding yields also fell in the range of 0.32, 0.41 and 0.43 kJ/g VSadded, respectively. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.