In this study, grape seed oil was transesterified with methanol using sodium hydroxide as a catalyst to obtain grape seed oil methyl ester (GSOME). It was basically identified through Fourier transform infrared (FTIR) spectrum. The blends such as B10, B15, B20, and D100 were tested in a compression ignition diesel engine. The fuel properties were determined using American Society of Testing and Materials (ASTM) and the engine emission parameters were analyzed for those test fuels for different load at constant engine speed of 1,500 rpm. The experimental results show that all the blends provided significant reductions in NOx than D100.