Peanut shells are agricultural solid waste abundantly available in China, but most of them are discarded or burned off in stacks, causing the resource dissipation and environmetal pollution. In this work, polyurethane sponge/graphite oxide/boronic acid functionalized metal-organic framework (PU/GO/BA-MOF) was formed via a three-in-one strategy, and then applied for selective adsorption and effective separation of cis-diol containing luteolin (LTL) from peanut shell coarse extract. Our three-in-one strategy was based on specific cis-diol moieties recognition function and exceptional chemical stability of GO/BA-MOF, and the PU sponge possessed the advantages of convenient adsorption-desorption process owing to its reversible "shape memory" effect. Additionally, PU/GO/BA-MOF can attain the adsorption capacity towards LTL six times than non-cis-diol structure. There was no significant change for the adsorption capacity of the PU/GO/BA-MOF, and the frameworks of PU/GO/BA-MOF did not collapse after it was reused five tithes. Compared with first cycle, the adsorption capacity loss is approximately 10.73% at fifth cycles. Subsequently, peanut shell coarse extract (85% LTL) was twice purified by PU/GO/BA-MOF, and the purification product (98% LTL) was successfully evaluated by MS-HPLC analysis and antibacterial test. Purified LTL (second time) have exhibited more excellent antibacterial effect and less impure peaks than purified LTL (first time). Therefore, the novel PU/GO/BA-MOF materials provided the effective way for the utilization of peanut shell as a source of flavone containing cis-diol structure. (C) 2016 Elsevier B.V. All rights reserved.