In this study, computational fluid dynamics (CFD) was used to systemically analyze the movement of algae in a vortex flow field produced by up-down chute baffles. The average cell light/dark (L/D) cycle period, vertical fluid velocity, fraction of time the algae was resides in light zone and the L/D cycle period were investigated under different paddlewheel speeds and microalgal concentrations. Results showed that the L/D cycle period decreased but the vertical fluid velocity increased when the up-down chute baffles were used. The L/D cycle period decreased by 24% (from 5.1 s to 3.9 s), and vertical fluid velocity increased by 75% when up-down chute baffles were used with paddlewheel speed of 30 r/min. The probability of L/D cycle period of 3 s increased by 52% from 0.29 to 0.44 with the up-down chute baffles. This led to approximately 22% increase in biomass yield without changing the paddlewheel speed. (C) 2016 Elsevier Ltd. All rights reserved.