High Rate Algae Ponds (HRAP) are the simplest way to grow microalgae biomass and an interesting alternative for wastewater treatment. In this work the performance of these systems was evaluated using anaerobically digested wastewater as culture medium. Two variables were studied in long-term mode: the carbon dioxide supply and the modification of the dilution rates. The results showed that CO2 supply increases the productivity but less than expected considering the potential biomass generation calculated based on the ratios of carbon to nitrogen of microalgae and wastewater. The assimilation into biomass only accounted for 57% of the inlet nitrogen under the best conditions because nitrification and volatilization reduced the availability of this element. The operation under short hydraulic retention times presented a more interesting performance with higher biomass productivities. The biomass produced was efficiently harvested with in a Dissolved Air Flotation (DAF) unit. (C) 2016 Elsevier Ltd. All rights reserved.