A series of gold-based catalysts supported by Fe-La-Al2O3 composite doped with various amounts of copper were prepared using a modified deposition-precipitation method. Investigations were carried out based on the reaction of the preferential carbon monoxide oxidation (CO-PROX) in H-2-rich stream and CO oxidation at low temperatures in an O-2-rich stream as a reference to examine the effects of copper on the catalytic performance. The high dispersion of gold nanoparticles in alumina-based support, the formation of Au-Cu alloy, and the close contacts of these neat or alloyed nanoparticles with CuO species, making Au partially positively charged, were examined with H-2-TPR, XPS, and HRTEM-FFT techniques. Gold catalysts were obtained with very promising performance for polymer electrolyte membrane fuel cell with positive results in activity, CO2 selectivity, and thermal stability and long-term storage and online stability. Although H-2 addition decreased the rate of CO oxidation over the studied gold catalysts, the activity and selectivity in CO2 formation were continuously improved with an increase in copper loading. (C) 2016 Elsevier B.V. All rights reserved.