Vegetable oils and biodiesel have emerged as strong alternative fuels worldwide. However use of new fuels in existing engines leads to issues such as wear of vital moving components, and fuel injection equipment (FIE). It is important to ensure that new alternative fuel does not affect the FIE adversely. In this experimental study, a non-firing engine FIE simulator test rig prototype was developed and 250 h endurance test of FIE was performed with an objective to ensure the long-term compatibility and durability of biofuel blends. The components of FIE such as plunger, nozzle needle, valve, and valve holder were investigated for wear. Test fuels used in this study were Karanja blends (K20, K5), Jatropha blends 020, J5), Biodiesel blends (B20, B5) and baseline mineral diesel in a non-firing engine FIE simulator. The compatibility of FIE with test fuels in terms of dimensional loss, weight loss and surface texture variations using optical microscopy before and after the endurance test was compared. Biodiesel blends showed relatively lower wear compared to mineral diesel however SVO blends showed no definite trend of the wear results compared to baseline mineral diesel. (C) 2016 Elsevier Ltd. All rights reserved.