Thermal conductivity and viscosity of the water-Fe3O4 nanofluid are investigated experimentally at volume concentrations of 0-4% and temperatures of 25-60 degrees C. For this purpose, an experimental setup is designed and developed to measure the thermal conductivity. The results reveal that both thermal conductivity and viscosity increase by increasing the volume concentration. Moreover, the temperature increment reduces the viscosity, while increases the thermal conductivity. It is shown that the thermal conductivity and viscosity have a nonlinear relationship with concentration. Moreover, the Hamilton Crosser and Einstein models are unable to predict the thermal conductivity and viscosity of the nano fluid properly. Based on the experimental data, models of thermal conductivity and viscosity are developed in terms of volume concentration and temperature using neural network. In order to train the neural network, three different methods belonging to three classes, namely resilient backpropagation, quasi-Newton and Levenberg-Marquardt, are examined, and among them, Bayesian regularization-based Levenberg-Marquardt approach is selected due to the best performance. The developed neural network is properly able to predict the thermal conductivity and viscosity of the nanofluid, and its results are found to be very consistent with the experimental ones. In addition, this model has a much better accuracy in comparison with the correlations obtained from regression. (C) 2016 Elsevier B.V. All rights reserved.