Arundo donax L. is a perennial plant that can substitute for traditional energy crops to produce biogas, reducing costs because of its high biogas yield per Ha cultivated and low agronomic and energetic inputs. Nevertheless, Arundo donax biomass needs to be ensiled to be preserved and used. Because no full-scale data exist about A. donax ensilage and the effect of this process on potential biogas production, in this work two different ensiling techniques, i.e. trench and silo-bag ensiling, were performed at full scale, and the processes studied for 200 days. Results obtained indicated that A. donax could be successful ensiled by using the two approaches. Ensilage proceeded by fermentation of organic acids already present in the biomass, i.e. malic and oxalic acids that were degraded, giving volatile fatty acid accumulation. This was different from corn ensiling characterized by starch fermentation to lactic acids. Biological processes determined a loss of the potential biomethane production, namely -20.1% and -7.6% for trench and silo-bag, respectively. Taking into consideration biomethane yield per Ha and ensilage losses, potential biomethane losses of 5000 Nm(3) CH4 Ha(-1) for trench silage and of 2000 Nm(3) CH4 Ha(-1) for silo bag, were estimated, respectively. Nevertheless, taking into consideration the higher biomass and biomethane yields Ha(-1) in comparison with the other energy crops, A. donax still remained more efficient and cheaper than traditional energy crops in producing biogas. (C) 2016 Elsevier Ltd. All rights reserved.