In our previous study, a bionic system simulating the cardiovascular system was built to realize a countercurrent multistage microextraction. However, further study on the hydrodynamics and mass transfer performances of such a system is still needed. In this study, a theoretical model was first established to describe the relationship between the pressure drop and the flow rate. The flow resistance, including the effect of the two-phase interface, was investigated based on the theoretical model and experimental data. The mass transfer coefficient was then investigated by experiments and computational fluidic dynamics (CFD) simulation. The overall mass transfer coefficient was found positively correlated with the slug velocity. Both the aqueous phase mass transfer coefficient and the organic phase mass transfer coefficient were obtained by CFD simulation. A correlation equation was established to calculate the Sherwood number, and it is proved to be applicable in both the aqueous and the organic phases.