Dendritic-linear-dendritic triblock copolymers have attracted much attention due to their unique topology and special features. However, facile methods are still necessary to synthesize uniform dendritic-linear-dendritic copolymers. Here, we report an effective strategy for the synthesis of a temperature- and pH-responsive dendritic linear dendritic poly(amido amine)-poly(N-isopropylacrylamide)-poly(amido amine) (PAMAM-PNIPAM-PAMAM), and evaluate the temperature- and pH-responsive properties of PAMAM-PNIPAM-PAMAM.