Many types of slippery liquid-infused porous surfaces ('SLIPS') can resist adhesion and colonization by microorganisms. These 'slippery' materials thus offer approaches to prevent fouling on commercial and industrial surfaces. However, while SLIPS can prevent fouling on surfaces to which they are applied, they can currently do little to prevent the proliferation of non-adherent organisms. Here, multi-functional SLIPS are reported that address this issue and expand the potential utility of these materials. The approach is based on the release of antimicrobial agents from the porous matrices used to host the infused oil phases. It is demonstrated that SLIPS fabricated from nanoporous polymer multilayers can prevent colonization and biofilm formation by four common fungal and bacterial pathogens, and that the polymer and oil phases comprising these materials can be used to sustain the release of triclosan, a model antimicrobial agent, into surrounding media. This approach improves the inherent anti-fouling properties of these materials and endows them with the ability to kill non-adherent pathogens. This strategy has the potential to be general; the strategies and concepts reported here will enable the design of SLIPS with improved anti-fouling properties and open the door to new applications of slippery liquid-infused materials that host or release other active agents.