Bi-compartmental 3D scaffolds for the co-culture of intervertebral disk cells and mesenchymal stem cells

Yun-Min Kook; Young Mi Kang; Seong Hwan Moon; Won-Gun Koh

Journal of Industrial and Engineering Chemistry, Vol.38, 113-122, June, 2016

DOI10.1016/j.jiec.2016.04.013 Export Citation

Bi-compartmental 3D scaffolds for the co-culture of intervertebral disk cells and mesenchymal stem cells

Yun-Min Kook, Young Mi Kang¹, Seong Hwan Moon^{1, †}, and Won-Gun Koh^†

Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea
¹Department of Orthopaedic Surgery, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea

E-mail:,

The combination of electrospinning and the subsequent gelation of alginate produced bi-compartmental hydrogel consisting of a nanofiber-incorporated hydrogel matrix domain and a bare alginate hydrogel domain. The co-culture system was prepared by placing intervertebral disk (IVD) cells in the bare alginate hydrogel and human bone marrow mesenchymal stem cells (hMSCs) in the nanofiber-incorporated hydrogel. Real-time polymerase chain reaction (PCR), western blotting, immunofluores-cence staining, and sulfated glycosaminoglycan assays revealed that the co-cultured groups produced more collagen type II, aggrecan, glucose transporter-1 (GLUT-1), and glycosaminoglycans (GAG) than the single-cultured hMSCs, confirming the enhanced differentiation of hMSCs in the co-culture system. It is expected that our bi-compartmental 3D scaffold can be applied to heterotypic co-culture systems for the study of various cell.cell interactions.

Keywords:Co-culture;Intervertebral disk cells;Mesenchymal stem cells;Bi-compartmental scaffold

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[1] Abbott A, Nature, 424, 870 (2003)

[2] Levenberg S, Langer R, Curr. Top. Dev. Biol., 61, 113 (2004)

[3] Battiston KG, Cheung JWC, Jain D, Santerre JP, Biomaterials, 35, 4465 (2014)

[4] Goers L, Freemont P, Polizzi KM, J. R. Soc. Interface, 11, 201400 (2014)

[5] Marimuthu M, Kim S, Anal. Biochem., 413, 81 (2011)

[6] Zervantonakis IK, Kothapalli CR, Chung S, Sudo R, Kamm RD, Biomicrofluidics, 5, 013406 (2011)

[7] Goubko CA, Cao XD, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 29, 1855 (2009)

[8] Kaji H, Camci-Unal G, Langer R, Khademhosseini A, BBA - Gen. Subj., 1810, 239 (2011)

[9] Folch A, Toner M, Annu. Rev. Biomed. Eng., 2, 227 (2000)

[10] Fukuda J, Khademhosseini A, Yeh J, Eng G, Cheng JJ, Farokhzad OC, Langer R, Biomaterials, 27, 1479 (2006)

[11] Hui EE, Bhatia SN, Proc. Natl. Acad. Sci. U. S. A., 104, 5722 (2007)

[12] Li JG, Li GC, Zhang K, Liao YZ, Yang P, Maitz MF, Huang N, Appl. Surf. Sci., 273, 24 (2013)

[13] Hwang IT, Jung CH, Choi JH, Nho YC, Langmuir, 26(23), 18437 (2010)

[14] Leisten I, Kramann R, Ferreiraa MSV, Bovi M, Neuss S, Ziegler P, Wagner W, Knuchel R, Schneider RK, Biomaterials, 33, 1736 (2012)

[15] Liu TJ, Lin BC, Qin JH, Lab Chip, 10, 1671 (2010)

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[17] Hussain A, Collins G, Yip D, Cho CH, Biotechnol. Bioeng., 110(2), 637 (2013)

[18] Santos MI, Unger RE, Sousa RA, Reis RL, Kirkpatrick CJ, Biomaterials, 30, 4407 (2009)

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[20] Lee J, Cuddihy MJ, Kotov NA, Tissue Eng. Part B: Rev., 14, 61 (2008)

[21] Le Maitre CL, Pockert A, Buttlet DJ, Freemont AJ, Hoyland JA, Biochem. Soc. Trans., 35, 652 (2007)

[22] Le Maitre CL, Freemont AJ, Hoyland JA, Arthritis Res. Ther., 9 (2007)

[23] Podichetty VK, Cell. Mol. Biol., 53, 4 (2007)

[24] Sakai D, Eur. Spine J., 17, S452 (2008)

[25] Freimark D, Czermak P, Int. J. Artif. organs, 32, 197 (2009)

[26] Richardson SM, Mobasheri A, Freemont AJ, Hoyland JA, Histol. Histopathol., 22, 1033 (2007)

[27] Kepler CK, Anderson DG, Tannoury C, Ponnappan RK, J. Am. Acad. Orthop. Surg., 19, 543 (2011)

[28] Ciapetti G, Granchi D, Devescovi V, Leonardi E, Greggi T, Di Silvestre M, Baldini N, Eur. Spine J., 21, S10 (2012)

[29] Richardson SM, Hoyland JA, Mobasheri R, Csaki C, Shakibaei M, Mobasheri A, J. Cell. Physiol., 222, 23 (2010)

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[31] Le Maitre CL, Baird P, Freemont AJ, Hoyland JA, Arthritis Res. Ther., 11 (2009)

[32] Peroglio M, Eglin D, Benneker LM, Alini M, Grad S, Spine J., 13, 1627 (2013)

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[36] Svanvik T, Henriksson HB, Karlsson C, Hagman M, Lindahl A, Brisby H, Cells Tissues Organs, 191, 2 (2010)

[37] Yang SH, Wu CC, Shih TTF, Sun YH, Lin FH, Spine, 33, 1951 (2008)

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[39] Allon AA, Aurouer N, Yoo BB, Liebenberg EC, Buser Z, Lotz JC, Spine J., 10, 1089 (2010)

[40] Allon AA, Butcher K, Schneider RA, Lotz JC, Cells Tissues Organs, 196, 99 (2012)

[41] Wu CC, Yang SH, Huang TL, Liu CC, Lu DH, Yang KC, Lin FH, Process Biochem., 47(6), 922 (2012)

[42] Yuan MT, Yeung CW, Li YY, Diao HJ, Cheung KMC, Chan D, Cheah K, Chan PB, Biomaterials, 34, 3948 (2013)

[43] Kim DH, Kim SH, Heo SJ, Shin JW, Lee SW, Park SA, Shin JW, J. Biosci. Bioeng., 108(1), 63 (2009)

[44] Vadala G, Studer RK, Sowa G, Spiezia F, Lucu C, Denaro V, Gilbertson LG, Kang JD, Spine, 33, 870 (2008)

[45] Naqvi SM, Buckley CT, Tissue Eng. Part A, 21, 288 (2015)

[46] Guarino V, Alvarez-Perez M, Cirillo V, Ambrosio L, J. Bioact. Compat. Polym., 26, 144 (2011)

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[48] Li WJ, Tuli R, Huang XX, Laquerriere P, Tuan RS, Biomaterials, 26, 5158 (2005)

[49] Bron JL, Vonk LA, Smit TH, Koenderink GH, J. Mech. Behav. Biomed., 4, 1196 (2011)

[50] Chiba K, Andersson GBJ, Masuda K, Thonar EJMA, Spine, 22, 2885 (1997)

[51] Meretoja VV, Dahlin RL, Kasper FK, Mikos AG, Biomaterials, 33, 6362 (2012)

[52] Allon AA, Butcher K, Schneider RA, Lotz JC, Spine, 37, 813 (2012)

[53] Richardson SM, Knowles R, Tyler J, Mobasheri A, Hoyland JA, Histochem. Cell Biol., 129, 503 (2008)

[54] Urban JPG, Roberts S, Arthritis Res. Ther., 5, 120 (2003)

[55] Le Visage C, Kim SW, Tateno K, Sieber AN, Kostuik JP, Leong KW, Spine, 31, 2036 (2006)