화학공학소재연구정보센터
Journal of Microencapsulation, Vol.17, No.3, 363-372, 2000
Effect of lecithins on BCG-alginate-PLL microcapsule particle size and stability upon storage
Alginate-PLL microcapsules containing BCG were prepared by emulsification/ internal gelation of an alginate solution dispersed within a vegetable oil containing lecithins as emulsifiers. The lecithins studied were soy bean lecithin at 0.1, 0.5, 1 and 2% concentration; and dried egg yolk lecithin at 0.1, 0.25, 0.5 and 1%. The microcapsule particle size showed a dependence upon the amount and type of lecithin added to the oil. Increasing the emulsifying agent concentration was found to reduce particle size, from 50.9 mu m obtained when lecithins were not used in the emulsification step, to 13.9 mu m obtained when 1% dried egg yolk lecithin was employed. The encapsulated BCG was identified by the Difco TB stain set K, followed by observation under optical microscopy. Once prepared, microcapsules were freeze-fried using 5% trehalose as cryoprotectant in order to preserve their stability upon storage. The stability of the microcapsules was assayed over 12 months at room temperature, finding that alginate-PLL microcapsules a:ere stable up to 6 months. Moreover, in the case of microcapsules prepared with lecithins, a significant increase in particle size was observed, from 16.9 mu m at the beginning of the study to 25.2 mu m at 12 months storage.