Biodegradable microspheres of PLGA (75:25 and 50:50) containing Ciprofloxacin (CIPRO) were prepared by two different procedures based on: (i) solvent-evaporation, and (ii) evaporation-extraction of the organic phase. The encapsulation rates from the different formulations were quite variable, as well the release patterns of the drug from the microspheres. The evaporation-extraction method seems to be more efficient for the CIPRO encapsulation compared with the solvent evaporation method. The 50:50 PLGA composition released the drug faster and showed degradation signs after incubation in aqueous medium in both manufacturing methodologies.