Monodispersed hollow mesoporous silica nanoparticles (HMSNs) are successfully synthesized via a facile dual template method, in which poly(styrene-co-methyl methacrylate-co-methacrylic acid) (PS-PMMA-PMAA) particles are used as hard template for producing the hollow structure and cetyltrimethylammonium bromide (CTAB) used for introducing the mesopores in the silica shells. The obtained HMSNs possess uniform diameter and morphology, and the shell of which could be adjusted by changing the addition of silicon precursor. The synthesized HMSNs have been characterized by transmission electron microscopy (TEM) and nitrogen physisorption. Furthermore, the HMSNs are used as support for in-situ deposition of silver nanoparticles (Ag NPs) using n-butylamine as reducing agent for AgNO3 in ethanol. Significantly, Ag NPs were successfully supported in the HMSNs without any aggregation. The Ag-deposited HMSNs showed excellent dispersibility in ethanol and water, and their antibacterial activity against Escherichia coli (E. coli) ATCC 25922 and Staphylococcus aureus (S. aureus) ATCC 6538 have been demonstrated. Therefore, the unique nanostructure based on the HMSNs provided a useful platform for the fabrication of antibacterial agent with superior activity and accessibility. And also, it is expected to be a significant template for the synthesis of other novel nanostructures. (C) 2016 Elsevier Inc. All rights reserved.