The liquid crystalline (LC) organization formed through a complementary hydrogen-bonded nucleobase-pair of adenine (A)-thymine (T) has been studied in this research. The non-mesogenic compounds have been synthesized by using adenine and thymine with different lengths of normal alkyl chains. The mixing of the two non-mesogenic units together resulted in the formation of mesophases. The A-T base pair is found to play an important role in the formation of the LC phase, and create the functionalized core connected by hydrogen bonds to act like a mesogenic group.