Hemicelluloses dissolved in thermomechanical pulp (TMP) mill process streams can be used to manufacture high-value-added products. The molecular mass of the dissolved hemicelluloses is about 10 kDa. In some applications, it would be beneficial with larger molecules. It was therefore investigated whether it is possible to increase the size of hemicelluloses isolated by microfiltration and ultrafiltration from TMP process water using enzymatic treatment with laccase. No significant increase of the size of hemicelluloses was achieved, probably due to only a small percentage of lignin carbohydrate complexes in the process water. The molecular mass of lignin increased however markedly from a peak molecular mass of 1 kDa to about 60 kDa. Diafiltration was used to purify large molecules after the enzymatic treatment. Low-molecular-mass sugar and lignin molecules in the diafiltration permeate were concentrated by nanofiltration. The retention of sugars and lignin was about 98% during nanofiltration to a volume reduction of 80%. Average flux during nanofiltration was 491/m(2) h. (C) 2016 Elsevier B.V. All rights reserved.