Non-enzymatic glucose biosensor was developed by utilizing the electrochemically grown nanocages-augmented polyaniline nanowires (NCa-PANI NWs) on silicon (Si) substrate. The NCa-PANI NWs were comprehensively analyzed in terms of the composition, optical, structural and the morphological properties. The grown NCa-PANI NWs were distributed uniformly on the entire surface of Si substrate, which confirmed the formation of highly dense NCa-PANI NWs networks during the electrochemical oxidation. A series of sensing performances for NCa-PANI NWs electrode were investigated by current (I)-voltage (V), cyclic voltammetry (CV) and amperometry measurements. The sensing results revealed that fabricated non-enzymatic sensor showed an excellent response to glucose with a stable, reliable, and high sensitivity of similar to 156.4 mA mM(-1) cm(-2), good detection limit of similar to 0.657 mu M with correlation coefficient (R) of similar to 0.99493. The fabricated glucose sensor based on NCa-PANI NWs electrode exhibited significant electrochemical stability, good reproducibility and the selectivity. (C) 2016 Elsevier B.V. All rights reserved.