Evaluation of the Therapeutic Potential In vitro and In vivo of the SIS/PLGA Scaffolds for Costal Cartilage Regeneration

Se Rom Cha; Sun Ah Cho; Seon Eui Lee; Na Keum Jang; Sung Jun Cho; Jeong Eun Song; Gilson Khang

Macromolecular Research, Vol.24, No.5, 400-408, May, 2016

DOI10.1007/s13233-016-4065-x Export Citation

Evaluation of the Therapeutic Potential In vitro and In vivo of the SIS/PLGA Scaffolds for Costal Cartilage Regeneration

Se Rom Cha, Sun Ah Cho, Seon Eui Lee, Na Keum Jang, Sung Jun Cho, Jeong Eun Song, and Gilson Khang^†

Department of BIN Fusion Technology, Department of PolymerNano Science Technology, Chonbuk National University, 567 Baekje-daero, Deokjin, Jeonju, Jeonbuk, 54898, Korea

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In this study, porcine small intestinal submucosa (SIS) without immune response were used to make SIS/PLGA scaffolds with different SIS content i.e. 0, 10, 20, 40, and 80 wt%. Further, the attachment and proliferation of costal cartilage cells (CCs) in SIS/PLGA scaffolds were characterized. Glycosaminoglycan (sGAG) and collagen contents assay were conducted to verify the effects of SIS on extracellular matrix (ECM) formulation. The CCs specific gene expression was confirmed by polymerase chain reaction (PCR). In conclusion, the sGAG production was formed to be higher in the 20 wt% SIS/PLGA scaffolds, which showed enhanced CCs cell growth and proliferation compared with other scaffolds.

Keywords:small intestinal submucosa (SIS);glycosaminoglycan;costal cartilag (CCs);scaffolds

[References]

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